Digital PCR (dPCR) is a technique that divides the PCR reaction system into a large number of tiny reaction units (such as droplets, microchambers, etc.), ensuring that each unit contains only 0, 1 or a few target molecules. Then, each unit is independently amplified and the number of positive units is counted, thereby achieving absolute quantification of the target nucleic acid molecules.
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AD3207 Automated Droplet Digital PCR SystemOur droplet digital PCR system can do fully automated, high-sensitivity detection of single-copy genes with facilitating accurate quantification of pathogens and viral loads. Featuring 7... -
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Pilot1200 All-in-one Digital PCR InstrumentThe Pilot1200 is a globally first leading "sample-to-answer" fully automated, integrated digital PCR system, revolutionizing the workflow by combining complex nucleic acid extraction, reaction... -
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D3200 Droplet Digital PCR System （China Only）The D3200 semi-automatic digital PCR is our company's first commercial product. Thanks to its excellent performance, it has received wide recognition from the market. It consists of three separate...
FAQ
Q: What are the advantages of digital PCR compared to qPCR?
A: Core Advantages of Digital PCR over qPCR
The Five Core Advantages of Digital PCR
These advantages all stem from its fundamental principle of ""partitioning"" and ""endpoint absolute counting.""
1. Absolute Quantification Without a Standard Curve
2. Ultra-High Sensitivity and Rare Variant Detection
3. Superior Precision and Reproducibility
4. Greater Tolerance to PCR Inhibitors
5. Independence from Amplification Efficiency
Q: Digital PCR, qPCR, and blood culture for the comparison in the detection of bloodstream infections
A:
| Feature | Digital PCR | qPCR | Blood Culture |
| Core Principle | Endpoint absolute nucleic acid counting | Real-time relative nucleic acid quantification | Microbial growth and cultivation |
| Detection Target | Pathogen-specific nucleic acids (DNA/RNA) | Pathogen-specific nucleic acids (DNA/RNA) | Viable, culturable microorganisms |
| Turnaround Time | Fast (Our TAT 3 hours) | Fast
But Filmarray BCID2 is based on positive blood culture |
Slow (2-5 days, avg. 24-48 hrs) |
| Sensitivity | Exceptionally High (single-copy level) | High | Lower (requires >10-100 CFU/mL) |
| Quantification | Absolute (copies/mL) | Relative (Ct value, needs standard curve) | Semi-quantitative (time-to-positivity, colony count) |
| Effect of Antibiotics | No Effect (detects nucleic acid from dead/live organisms) | No Effect (detects nucleic acid from dead/live organisms) | Severe Impact (prior antibiotics can cause false negatives) |
| Inhibitor Tolerance | Excellent, highly tolerant to blood inhibitors | Weaker, susceptible to blood inhibitors | N/A, but subject to microbial inhibition |
| Pathogen Coverage | Targeted, requires known sequence | Targeted, requires known sequence | Broad-spectrum, unbiased, can detect unknown/rare pathogens |
| Primary Clinical Value | Early, precise, quantitative diagnosis | This technology is most widely applied in scenarios based on positive blood culture samples. | Definitive diagnosis & therapeutic guidance (Gold Standard, Slow) |
As one of the leading digital PCR manufacturers and suppliers in China, we offer a wide range of products with superior quality. Please feel free to wholesale custom made digital PCR from our factory. Contact us for more details.
Multiplex Digital PCR, digital pcr multiplex, digital pcr
